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Western blotting Cell lines were grown on 6 plates, treated as indicated, and lysed in NP40 lysis buffer, (1% v/v NP40, 10 mM Tris.Cl pH8, 150 mM NaCl, 1 mM EDTA, 1 mM DTT) supplemented with Protease/Phosphatase Inhibitor Cocktail (100X) (Cell Signalling Technology #5872)