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Most proteins are not stable in acidic pH, so you will want to adjust your pH back to the desired value using a concentrated base such as sodium hydroxide before using your elution buffer
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Furthermore, these low-resolution techniques were used long before the scientific community fully accepted the concept of biologically active proteins lacking a fixed 3D structure, and were instrumental in laying the groundwork for this vital discovery, played a crucial role in the discovery of the protein intrinsic disorder phenomenon and, later, offered tremendous help with establishing the IDP/IDR field
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