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Mouse cerebellar astrocytes protect cerebellar granule neurons against toxicity of the polybrominated diphenyl ether (PBDE) mixture DE-71

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To precipitate proteins, 20 μl of plasma or 50 μl of working standard was mixed with 90 μl of methanol containing internal standards (200 ng/ml acetaminophen-d4 and 1000 ng/ml acetaminophen-sulfate-d3)