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Percentages of proliferative (BrdU + ) (n = 6 technical replicates representative of 2 independent experiments) ( a ) and apoptotic (Annexin V + ) (n = 3 technical replicates representative of 3 independent experiments, **p = 0.0042) ( b ) cells were determined

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The cancer cells were preincubated with NAC and subsequently treated with NP4 showed a reduced number of TNF-α secreted from the macrophages, suggesting that macrophage activation is associated with the NP4-induced oxidative stress