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Interestingly, when FOXO4 expression was silenced in TM3 cells using siRNA prior to H2O2 treatment, cell viability decreased, and apoptosis increased, despite elevated p53 levels

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Moreover, participants were asked not to change routine dietary and physical activity habits during the study to eliminate the probable confounding impacts of dietary/physical activity alterations on the study results, as well as not to use pro/pre/synbiotic-supplemented food products during the intervention period