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Briefly, to verify the targeted binding between miR-29b-3p and the GGCT gene, a 2000-bp DNA fragment (derived from the transcription start site of the GGCT gene) was inserted into the pmir-GLO-Basic vector to construct the wild-type pmir-GLO-GGCT-3′ UTR reporter plasmid (wt) and the mutant pmir-GLO-GGCT-3′ UTR reporter plasmid (mut)

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[DOI] [PMC free article] [PubMed] [Google Scholar] 66.Treberg JR, Brosnan ME, Watford M, Brosnan JT