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Flow cytometry analysis The proportion of M1 (CD45 + CD11b + Ly6G - F4/80 + CD206 - ) and M2 (CD45 + CD11b + Ly6G - F4/80 + CD206 + ) in RAW264.7 cells and mouse spleen macrophages were analyzed by flow cytometry

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PCOS women with insulin resistance have a higher accumulation of visceral fat, a surplus of androgens and experience more severe clinical symptoms, including hirsutism, acne, and ovulatory disorder, in contrast to PCOS women lacking insulin resistance [54]